Journal: Archives of microbiology

Article Title: Isolation and identification of a bacteriocin with antibacterial and antibiofilm activity from Citrobacter freundii

doi: 10.1007/s00203-012-0793-2

Figure Lengend Snippet: Strains used in the study

Article Snippet: In order to investigate whether the predicted immunity gene, from C. freundii ATCC 43864 plasmid pCfc1, is capable of providing resistance to the antimicrobial activity of ColA-43864, bacteriocin-sensitive wild-type E. coli S17-1, C. freundii NCTC 9750, and C. freundii ATCC 8090 were transformed by electroporation with pCfc-8A (pCfc1 with a mutated colA - 43864 gene).

Techniques: Mutagenesis, Plasmid Preparation, Functional Assay

Journal: Archives of microbiology

Article Title: Isolation and identification of a bacteriocin with antibacterial and antibiofilm activity from Citrobacter freundii

doi: 10.1007/s00203-012-0793-2

Figure Lengend Snippet: Plasmids used in the study

Article Snippet: In order to investigate whether the predicted immunity gene, from C. freundii ATCC 43864 plasmid pCfc1, is capable of providing resistance to the antimicrobial activity of ColA-43864, bacteriocin-sensitive wild-type E. coli S17-1, C. freundii NCTC 9750, and C. freundii ATCC 8090 were transformed by electroporation with pCfc-8A (pCfc1 with a mutated colA - 43864 gene).

Techniques: Plasmid Preparation, Mutagenesis, Expressing

Journal: Archives of microbiology

Article Title: Isolation and identification of a bacteriocin with antibacterial and antibiofilm activity from Citrobacter freundii

doi: 10.1007/s00203-012-0793-2

Figure Lengend Snippet: Transformation of E. coli with pCfc1. Overnight cultures of wild-type E. coli S17-1, E. coli S17-21pA, and E. coli-8A were spotted on a lawn of sensitive C. freundii NCTC 9750. Antimicrobial activity is seen by the formation of a zone of inhibition around the point of inoculation

Article Snippet: In order to investigate whether the predicted immunity gene, from C. freundii ATCC 43864 plasmid pCfc1, is capable of providing resistance to the antimicrobial activity of ColA-43864, bacteriocin-sensitive wild-type E. coli S17-1, C. freundii NCTC 9750, and C. freundii ATCC 8090 were transformed by electroporation with pCfc-8A (pCfc1 with a mutated colA - 43864 gene).

Techniques: Transformation Assay, Activity Assay, Inhibition

Journal: Archives of microbiology

Article Title: Isolation and identification of a bacteriocin with antibacterial and antibiofilm activity from Citrobacter freundii

doi: 10.1007/s00203-012-0793-2

Figure Lengend Snippet: Expression of a predicted bacteriocin immunity gene in ColA-43864-sensitive bacteria. a Bacteriocin antimicrobial activity assay. Crude ColA-43864-containing extracts were spotted on microbial lawns of C. freundii NCTC 9750, C. freundii ATCC 8090, and E. coli S17-1, as well as transformants harboring plasmids: pMQ124 (empty vector), pCfc1-8A (colA-43864 defective, intact immunity gene), or pMQ345 (vector + predicted immune gene from pCfc1). Antimicrobial activity is seen by the formation of a zone of inhibition, and the bacteriocin was spotted. b Effect of bacteriocin on E. coli harboring the immune gene. Planktonically grown E. coli S17-pMQ345 and an empty vector control E. coli S17-pMQ124 (~107 CFU ml−1 of each strain) were incubated for 30 min with PBS (black bars), 87 μg protein extracted from empty vector control E. coli S17-pMQ124 (white bars), and 37 μg protein derived from E. coli S17-pMQ348 (gray bars). Cell viability was measured at time 0 and following incubation. Each value represents the mean of 3 experiments. Error bars are shown as one-standard deviation

Article Snippet: In order to investigate whether the predicted immunity gene, from C. freundii ATCC 43864 plasmid pCfc1, is capable of providing resistance to the antimicrobial activity of ColA-43864, bacteriocin-sensitive wild-type E. coli S17-1, C. freundii NCTC 9750, and C. freundii ATCC 8090 were transformed by electroporation with pCfc-8A (pCfc1 with a mutated colA - 43864 gene).

Techniques: Expressing, Activity Assay, Plasmid Preparation, Inhibition, Incubation, Derivative Assay, Standard Deviation